ZFIN Image: Leino et al., 2023, Fig. 1.

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Figure Caption

Fig. 1.

Expression of Zbtb16 protein and zbtb16a/b mRNA in the zebrafish hindbrain. (A,B) Wholemount fluorescence micrographs showing staining with anti-Zbtb16 antibody (see Materials and Methods) at 14 hpf and 24 hpf. (C,F,I) Immunofluorescent staining with anti-Zbtb16 antibody at 14, 16 and 24 hpf. C and F show slices from confocal z-stack, I shows sum projection through z-stack. n≥5 embryos per stage. (D,E,G,H,J-O) HCR for zbtb16a and zbtb16b at 14, 16, 24, 30 and 44 hpf. Sum projections through the z-stack. n≥5 embryos per stage. The Tg[egr2b:H2B-Citrine] line expresses Citrine in rhombomeres (r) 3 and 5. Arrows indicate boundaries between high and low levels of Zbtb16 protein or zbtb16a/b mRNA expression. MB, midbrain; MHB, midbrain-hindbrain boundary; op, otic placode; ov, otic vesicle. Scale bars: 50 µm.

Acknowledgments

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