Figure 2

Figure 2

Homozygous sox18 mutants show subtle but statistically significant defects in thoracic duct (TD) formation. (A) Confocal trunk images representing wt (+/+) and sox18^sa12315 homozygous mutant larvae (−/−) in the Tg(lyve1b:DsRed) line at 5dpf. Large and small white arrowheads point to TD+ segments (of typical or thinner aspect, respectively) while asterisks indicate the absence of TD. (B) The graph reports the mean number of TD+ segments, counted along 10 consecutive trunk segments, together with the Standard Error of the Mean (SEM), in all analyzed embryos of the three genotypes (wt: sox18^+/+, het: sox18^+/−, hom: sox18^−/−). Data were gathered in three independent experiments, and each symbol represents the number of TD+ segments of a single analyzed larva. n = number of larvae, ** = p < 0.01. TD = thoracic duct; DA = dorsal aorta; PCV = posterior cardinal vein. Lateral view, anterior to the left.