ZFIN Image: Meier et al., 2023, FIGURE 7

Image

Figure Caption

FIGURE 7

Initial screening of human as well as zebrafish CK1δ and ε with selected inhibitors. The selected inhibitors G2-1, G2-2, G2-3, G2-4 and G2-6 were screened at a concentration of 20 µM in in vitro kinase assays with GST-humCK1δ^TV1, GST-CK1ε, His-DrCK1δA, His-DrCK1δB and His-DrCK1ε. The residual kinase activity [%] was determined by measuring the radioactively labelled γ-phosphate incorporation into the substrate α-casein by Cherenkov counting. The results of the inhibitor-treated samples were normalized against the DMSO control, plotted against the respective inhibitor and are presented ±standard deviation.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Pharmacol