Fig. 4

Fig. 4 Activator-blocker model: Pou5f3 and Nanog oppose each other effects on H3K27 acetylation and chromatin accessibility on a fraction of enhancers.

a Summary profiles of H3K27ac mark in the WT and MZnps *-data from⁵. b Summary profiles of H3K27ac histone mark in the wild-type and single mutants, in four types of TdARs: 1.PN, 2.P, 3.N, 4.-. Note the opposite effects of Pou5f3 and Nanog on H3K27 acetylation on the 2.P and the 3.N groups. c Pou5f3 and Nanog have the opposite effects on H3K27ac on 2.P p + n- and 3.N p-n+ antagonistic enhancers. d Pou5f3 and Nanog have the opposite effects on chromatin accessibility on 2.P p + n- and 3.N p-n+ antagonistic enhancers. e,f Genomic browser views show (from bottom to top) motif occurrence, TF binding, H3K27ac and ATAC-seq in the indicated genotypes. e All three TFs bind to sox:pou motif on her3 2.P p + n- antagonistic enhancer (blue shading). f Pou5f3 and Nanog, or all three factors bind nanog motifs on morc3b 3.N p-n+ antagonistic enhancers (yellow shading). g Schematic illustration of activator-blocker model (explanations in the text). Source data are provided as a Source Data file 1 (a–f).