Fig. 4
Fig. 4. CYP1A, ROS, and cell death in the wild-type (AB strain) zebrafish larvae exposed to PSMPs in the presence of 5 and 10 ppm BaA. (a) The EROD induction by the live-imaging assay in larvae. (b) The EROD activity. (c) The fluorescence images of larvae stained by H2DCFDA. The white arrow pointed to the heart region. (d) ROS generation in the heart region of the larvae. (e) The fluorescence images of larvae stained by AO. (f) Cell death the in the heart region of the larvae. The white rectangular boxes indicated the heart region for analysis of cell death. The statistical difference compared to the control group: *p < 0.05 and ***p < 0.001. The statistical difference compared to the BaA treatment group: #p < 0.05, ##p < 0.01, and ###p < 0.001. Scale bar: 500 μm.