Figure 2.

Figure 2.

A loss of MR impacts macrophage production and distribution during development. (A) Transcript abundance of csf1ra in FACS-sorted macrophages from WT and MRKO larvae at 48 hours postfertilization (hpf), determined by qPCR (n = 3). (B) Number of mpeg+ macrophages in the yolk at 30, 50, and 72 hpf in WT and MRKO embryos/larvae at different stages of development (n = 3-5). (C) Representative images of mpeg+ macrophage (magenta) populations within the yolk of Tg(mpeg:mCherry);nr3c2^+/+ (WT) or Tg(mpeg:mCherry);nr3c2^inr11/inr11 (MRKO) larvae at 30, 54, and 72 hpf. Scale bar: 100 µm. Bars show mean ± SEM of data pooled from 3 different experiments (B; each data point representing a single embryo/larva). Data were analyzed using a 2-way ANOVA (Holm-Sidak post hoc test). Statistical significance is indicated by *P ≤ .05, **P ≤ .01.