FIGURE 6

FIGURE 6

Alteration of the noradrenaline pathway in GHes-fed zebrafish. Control- or GHes-fed zebrafish (7 days) were exposed to alarm substance stress. (A–C) The fish brains were excised 5 min after stress, and the contents of noradrenaline (A), dopamine (B), and serotonin (C) in the brain were estimated using HPLC. (D and F) Fish brains were excised 15 min after stress. Brain lysates were subjected to Western blotting with anti-Th (D) and anti-phospho-ERK (F) antibodies. Actin and ERK were used as internal controls. Quantitative analyses of the intensities of the protein bands were conducted, and the results are presented as Th/β-actin and p-ERK/ERK. n = 8. (E) Fish brains were excised 15 min after stress induction. c-fos mRNA levels in the zebrafish brains were assessed using real-time PCR. Each level of gene expression in GHes-fed zebrafish was relative to that in control. n = 10. n.s., not significant. Results are shown as means ± standard deviation.