Fig. 4

Fig. 4

Efflux activity of zebrafish Abcb4 in the larval brain vasculature a Diagram of the intravascular injection of rhodamine123 experiment. Rhodamine123 (green) was injected into the cardinal vein of Tg[flk1:EGFP] and Tg[flk1:EGFP]; abcb4^−/− embryos at 3 dpf and allowed to circulate for 1.5 h before imaging. b Representative images of the dorsal view of the larval brain after rhodamine123 injection showed the level of rhodamine123 accumulation in the brain area. c Quantification of normalized rhodamine123 intensity in the brain area of Tg[flk1:EGFP] and Tg[flk1:EGFP]; abcb4^−/− embryos. Data are expressed as mean ± SD from three independent experiments (black dots). N numbers (gray dots) indicate the total number of embryos across the three independent experiments. P-values were calculated using two-way ANOVA