ZFIN Image: Park et al., 2023, Fig. 3

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Figure Caption

Fig. 3

Zebrafish Abcb4 protein localizes to blood vessels in the zebrafish brain. Brain tissues of WT a and abcb4 knockout b adult zebrafish, as a negative control, were stained with anti-Pgp antibody F4 (red) as described in the Materials and Method section. Bar = 30 μm. c F4 antibody staining of whole adult zebrafish. Bar = 3 mm. Positive staining (red) was noted in the forebrain d, intestine e, and a subset of renal tubes or collecting ducts in the kidney f. g The F4 positive staining (red) in the brain colocalized with flk1:GFP positive cells (green). Fluorescence channels were interrogated individually and merged in. Nuclei were stained with DAPI (blue). Bar = 300 μm for (d, e), 200 μm for (f), and 50 μm for (g)

Acknowledgments

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