Fig. 2

Hypoxia-induced BBB injury is related to oxidative stress. (A) Quantification of MDA content in the head of zebrafish. N = 150 fish per group. Mean ± SD, Student's t-test, ***p = .0002. (B) 4-HNE and β-tubulin proteins were measured in zebrafish head by Western blot. Mean ± SD, Student's t-test, *p = .0301. N = 100 fish per group. (C) Representative images of bEnd.3 cells stained with ROS assay kit and Hoechst. Mean ± SD, Student's t test, **p = .0011, scale bar: 100 μm. (D) Immunofluorescence staining of CLDN5 and 4-HNE. Mean ± SD, Student's t-test, *p = .0316. (E) Typical bands of 4-HNE and β-tubulin proteins in bEnd.3 cell by Western blot. Mean ± SD, Student's t-test, *p = .0189.