Figure 2

Figure 2

MMP-13 immunofluorescence staining in different skin compartments. Stratum spinosum and granulosum, SS + SG; stratum basale, SB; and dermis. (a) MMP-13 antibody staining in control (top panel) and two CIPN patients (middle panel at 5 weeks, and lower panel at 31 weeks post CIPN diagnosis). White boxes depict zoomed-in regions shown in the second column. MMP-13 positive cells are present in the basal (SB) layer (white arrows). The white arrowhead in the lower left panel points to MMP-13 positive cells in the SS + SG layer. The yellow arrows depict increased dermal expression in the CIPN skin. Insets with Hoechst33342 stain show darker nuclei in the CIPN patient skin compared with the control subject skin. The scale in unlabeled images is identical to the panel on the left. (b) Quantification of MMP-13 expression in full-thickness skin. (c) Quantification of MMP-13 expression in sub-compartments (SG + SS vs. SB vs. dermis).