FIGURE 5

FIGURE 5

Hematopoietic‐promoting activity of hydroxysafflor yellow A (HSYA) on mice bone marrow nucleated cells: (A) experimental protocol of bone marrow nucleated cells (BNCs) extraction and drug treatment; (B) ATP content of control and N,N,N′,N′‐tetrakis(2‐pyridylmethyl)ethylenediamine (TPEN)‐treated BNCs with or without HSYA pretreatment; (C) representative images and quantitative result of hematopoietic colony‐forming unit (CFU) assay. (D and E) Western blot images and quantitative results showing P53 and cleaved‐Caspase3/Caspase3 expression of control and TPEN‐treated BNCs with or without different dosages of HSYA; (F) QPCR results showing the expression of P53 in BNCs with indicated treatment; (G) fluorescence intensity and quantitative result of reactive oxygen species detected by flow cytometry. The dosage of TPEN is 7.5 μM in all groups; the dosages of HSYA are 50 μg/ml in (C) and 12.5 μg/ml in (F and G), and as listed in (B) and (D). BFU‐E, burst‐forming unit‐erythroid; GM, granulocyte–monocyte; GEMM, granulocyte‐erythrocyte‐macrophage‐megakaryocyte. Scale bar in (C), 50 μm; *p < 0.05; **p < 0.01.