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Fig. 4
Fig. 4. Stacked 2D contour maps depicting the two-photon excited fluorescence intensities of calcite crystals grown in 25 mM CaCl2 mineralizing solution in presence of 150 ?g/ml unlabeled and 200 nM concentration of labeled dOtol1 (A), hOtol1 (B) and 250 nM free ATTO-488 NHS ester control (C) taken at several z-positions. The average power of the incident laser beam was set to 250 ?W (?ex = 800 nm). The color bar corresponds to the fluorescence intensity.
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Reprinted from Biochimica et biophysica acta. General subjects, 1867(5), Bielak, K., Benkowska-Biernacka, D., Ptak, M., Stolarski, J., Kalka, M., O?yhar, A., Dobryszycki, P., Otolin-1, an otolith- and otoconia-related protein, controls calcium carbonate bioinspired mineralization, 130327, Copyright (2023) with permission from Elsevier. Full text @ BBA General Subjects