Fig 9 Label retaining cell assays (LRC) to identify spermatogonial stem cells.
A: 32 dpf fish were treated with BrdU three times for about 16 hours per treatment (overnight) followed by a 25 day chase where fish were in water with no BrdU. At the end of the chase period, fish were fixed and processed for BrdU detection. B-F: Wild-type testes all had LRCs (B-C, arrows) with median percentage of 9.34% out of the total spermatogonia (N = 8 fish) (F). Four out of eight mutant (