Fig. 7.

Fig. 7.

Chronic hyperglycemia can be achieved in adults using NTR2.0 fish. (A) Timeline of the chronic MTZ treatment trial. (B) Blood glucose readings of individual adult zebrafish from the following groups: negative controls (wild type, no MTZ, -ve. ctrl), MTZ controls (wild type with MTZ, MTZ ctrl) and three experimental groups (ins:mCherry NTR2.0^ir2018 fish treated with MTZ as outlined in A, Expt. 1-3). All fish in the experimental groups became hyperglycemic. Owing to the limitation of the blood glucose meter used, 500 mg/dl is the maximum blood glucose reading that can be captured. (C) Percentage change in body weight over the course of the trial for individual fish in control and experimental groups. The dotted line represents no change in body weight. (D-M) Representative images of female and male fish from control and experimental groups. Scale bars: 1 cm. N.S., nonsignificant; ***P<0.05 (unpaired two-tailed Student's t-test). FBG, fasting blood glucose; MTZ, metronidazole.