Fig. 4

Fig. 4 Knockdown Bmp8a promotes adipogenesis.

a, b After induction of adipogenic differentiation, Bmp8a knocked-down 3T3-L1 cells (LV-shRNA-Bmp8a#1) and control cells (Mock and LV-shRNA-scrambled) were stained with Oil Red O and subjected to OD₄₉₂ quantifications (n = 3). Scale bar = 20 µm. c–f On the day after induction as indicated, expressions of adipogenic markers (Cebpα, Pparγ, and Fasn) were examined at the mRNA levels by qPCR (n = 3). g–i On the day after induction as indicated, the protein levels of PPARγ and C/EBPα were detected by Immunoblot (g). Protein expression levels were quantified using ImageJ software and normalized to the amount of β-actin (h, i, n = 3). Data were representative of at least three independent experiments. Data were analyzed by One-way ANOVA and presented as mean ± SD (**p < 0.01, ***p < 0.001).