ZFIN Image: Suzuki et al., 2023, Figure 4

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Figure Caption

Figure 4

Wright–Giemsa staining of erythroid cells from wild-type and klf1-klf17 mutant embryos. (A, B) Wright–Giemsa staining at 52 hpf. Erythroid cells in the klf1-klf17 mutant (B) had larger nuclei and more basophilic cytoplasm than wild-type cells (A) at 52 hpf. Scale bar, 10 μm (A). Genotyping was performed by genomic PCR using locus-specific primers. (C) Scatter plots of the nucleus-to-cytoplasm (N:C) ratio in the wild-type (n = 6) and the klf1-klf17 mutant (n = 4) at 52 hpf. The N:C ratio in the klf1-klf17 mutant was larger than that in the wild-type. The central horizontal lines indicate the mean values. The data shown are the mean ± standard deviation (SD). Each wild-type and klf1-klf17 mutant sample contained 10 cells. P < 0.001 was considered significant.

Acknowledgments

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