Figure 5

Figure 5

(A) Cropped region of the muscle fiber imaged using FPALM. The purple line shows the path along which the intensities of the pixels are measured. Purple circles show positions of intensity peaks. (B) Plot of pixel intensities vs. distance along the line shown in A. Purple dots show the positions of maximum intensity. The distance between intensity maxima represents the distance between the t-tubules. The apparent width of the t-tubule (represented by the green dotted line) was obtained by measuring the full width at half maximum of the intensity peak, represented by the red dotted line.