ZFIN Image: Drepanos et al., 2023, Figure 1

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Figure Caption

Figure 1

Principal Component Analysis reveals the effects of time of day, genotype, and Zeitgeber on variance between RNA-seq samples. (a) Average variance in expression values (y-axis) along PCs 2–4, with respect to time of day (x-axis) and genotype (red and black bars). Error bars are the SEM for the four biological replicates. PC1 was determined to be a likely technical artifact (Supplemental Figs. S1–3). (b) Example genes whose expression variance was positively or negatively correlated with PCs 2–4. The Pearson correlation coefficient of the gene’s expression variance with the PC is shown in parentheses.

Acknowledgments

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