ZFIN Image: Farre et al., 2023, FIGURE 12

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Figure Caption

FIGURE 12

Expression of si:busm1 in larval zebrafish. (A–D) Projections of representative whole, imaged eyes. (E–F”’) Single z slices obtained from the same preparations. Note reduced expression domain of lws2 (cyan), and expanded expression domains of lws1 (magenta) in T3-treated (B,D) vs. controls (A,C); the si:busm1 (yellow) expression domain appears greatly reduced in the treated condition (D). (E’–E”’) Enlarged images of region within box in panel (E). (E’) All imaging channels merged. (E”)si:busm1(E”’)lws2(E””)lws1(F’–F””) Enlarged images of region within box in panel (F). (F’) All imaging channels merged. (F”)si:busm1(F”’)lws2(F””)lws1(G–J) Projections of whole embryo heads. (K) qPCR quantification of gngt2a transcript abundance in pooled samples of whole larvae, n = 5 biological replicates, p = 0.244. (L) 3D fluorescence intensity quantification, n = 5 (DMSO), 4 (T3), p = 0.056 (t-test). D, dorsal, T, temporal. n.s., not significant.

Acknowledgments

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