ZFIN Image: Sun et al., 2023, Figure 2

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Figure Caption

Figure 2

Morpholino-targeted knockdown and targeted knockout of lmo4a. (A) Bands of 483 bp and 324 bp represent correctly spliced and artificially mis-spliced lmo4a mRNA. Colored boxes indicate exons. Arrows signify primers used for RT-PCR and the black bar depicts lmo4a MO. (B) Protein alignment including the truncated protein generated by lmo4a MO. (C) RT-PCR of control embryos and those injected with 2 pg lmo4a MO showing effective but incomplete disruption of splicing (m, 100 bp ladder marker). (D–G) PCR and Sequencing analysis showing the lmo4a knockout efficiencies of the four gRNA targets.

Acknowledgments

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