Figure 4

Figure 4

Surface electrical impedance myography detects age-related muscle changes in wildtype zebrafish. sEIM parameters (phase, reactance, and resistance) were assessed at a range of frequencies (1 kHz–1 MHz) in the epaxial caudal muscles of young (8 months, ~20% lifespan; n = 17) and aged zebrafish (36 months, ~85% lifespan; n = 17). Multifrequency graphs are shown for 3 impedance parameters: (A) phase, (B) reactance, and (C) resistance. Single frequency analyses at (D) 2 kHz phase (p < 0.000001, q = 0.000002), (E) 2 kHz reactance (p = 0.000004, q = 0.000006), (F) 2 kHz resistance (p = 0.000867, q = 0.000683), (G) 50 kHz phase (p = 0.000252, q = 0.000265), (H) 50 kHz reactance (p = 0.006081, q = 0.003192), (I) 50 kHz resistance, (J) 250 kHz phase (p = 0.044723, q = 0.020125), (K) 250 kHz reactance, (L) 250 kHz resistance (p = 0.001384, q = 0.000872). * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001, **** p ≤ 0.0001.