Fig. 3

Fig. 3

A) Quantification of the protein array probed with CM from control (Mneo) or Bcl-xL overexpressing (Mxl90) melanoma cells. Fold expression of Mxl90 relative to Mneo cells is reported. B,D) qRT-PCR analysis of B) IL-8, IL-1β, IL-4 and TNFα and D) MCSF, CCL2 and CCL5 in Mneo, Mxl90 and Mxl7 cells. C) IL-8 and IL-1β protein secretion by ELISA in Mneo, Mxl90 and Mxl7 cells. E,F) CCL5 protein expression by E) Western blot analysis and F) ELISA in Mneo, Mxl90 and Mxl7 cells. HSP70 is shown as loading and transferring control. One representative western blot analysis out of two with similar results is reported. The numbers indicate densitometric analysis relative to control. B-F) The results represent the average ± SEM of three independent experiments, p-values were calculated between control and Bcl-xL overexpressing cells, *p < 0.05, **p < 0.01