Figure 3

Figure 3

Increase in Isl1-positive cells is likely due to misspecification rather than migration defects. 3D renders of timelapse imaged ctnnd2b^+/+(A-D) and ctnnd2b^+/− embryos (A’-D’) with highlighted ORR (insets). Embryos at 32 hpf, prior to appearance of neurons at ORR, are shown in (A,A’). Isl1:GFP-positive neurons start appearing at the ORR by 37 hpf (B,B’), and detected at 42.5 hpf (C,C’) and 48 hpf (D,D’). Insets show the ORR at the corresponding timepoints. By the end of recording only a few isl1:GFP-expressing cells are found in ctnnd2b^+/+ embryos compared to ctnnd2b^+/− embryos.