Figure 6.
Flow cytometry analysis of GFP-expressing DiI labeled MDA-MB-231 cells. (a–c) Histograms of GFP, CellTrace Violet and DiI fluorescence intensity distribution for the GFP-expressing DiI and CellTrace Violet labeled cells at 0 to 4 days in culture, indicated by green, red, blue, dark and light purple traces, correspondingly. The black trace in (A) corresponds to the histogram for MDA-MB-231 cells that do not express GFP that were used as a control (labeled as wild-type in the figure). The black trace in (B) and (C) corresponds to histograms for DiI-unlabeled GFP-expressing MDA-MB-231 cells used as a control.