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Fig. 5
Malformation severity scoring. (a, d, g, j) Dorsal, (b, e, h, k) ventral, or lateral (c, f, i, l) images of five days post fertilization (dpf) embryos stained with alcian blue (cartilage) and alizarin red (bone) showing the craniofacial malformation scale that were used to quantify the number of malformations at each severity shown in (m). (n) Bar graph of frequency of malformations following ethanol exposure. Statistics = Fischer's exact test for each control‐treatment group comparison. Scale bar = 0.2 mm
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