Fig. 3
Ethanol exposure reduces fli1:EGFP expression. (a?d) Fluorescent images of 26 hpf fli1:EGFP embryos exposed to control or the indicated concentration of ethanol from 6?24 hpf showing dose?dependent reduction of fluorescence. (e) Column graph showing fli1:EGFP fluorescence for each embryo (open circles). Black bars show mean fluorescence in relative fluorescence units (RFU) for each group ± SEM. Sample sizes: control n = 15; 1% ETOH n = 16; 1.25% ETOH n = 14; 1.5% ETOH n = 16. Statistic: two?tailed ANOVA with Tukey's multiple comparison's correction between all groups. *** = p < .001, **** p < .0001. Scale bar = 0.2 mm. RFU = relative fluorescence units. ETOH = ethanol