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Fig. 2
Plate reader data analysis. (a) Heatmaps show the area scan results for a single 96?well plate. Treatment groups were as follows: (rows A + B = Control, C + D = 1% ETOH, E + F = 1.5% ETOH, G + H = 2% ETOH. (b) For each well, 25 single regions were scanned in a 5 × 5 matrix. These 25 per?well reads were thresholded to remove background fluorescence and target fluorescence from the head (see methods). (c) Fluorescence that met or exceeded the threshold value were summed to generate a cumulative well measurement for each embryo. ETOH = ethanol
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