Fig. 6

Fig. 6 Fig. 6. TCC reduces type II collagen expression via DNMT1-dependent DNA methylation. (A) The CpG island in the human COL2A1 gene. The arrows refer to the primer pairs. (B) The CpG island in the zebrafish col2a1a gene. The arrows refer to the primer pairs; (C) Quantification of CpG island methylation in the human COL2A1 gene treated with TCC or an equal concentration of DMSO (0.025 %, V/V).; (D) Quantification of CpG island methylation in the zebrafish col2a1a gene treated with TCC or an equal concentration of DMSO (0.025 %, V/V).; (E) Quantification of CpG island methylation in the human COL2A1 gene exposed to TCC with or without the presence of DC_517 or CM-272. An equal concentration of DMSO (0.025 %, V/V) was used as control; (F) Quantification of COL2A1 expression in human chondrocytes exposed to TCC with or without the presence of DC_517 or CM-272. An equal concentration of DMSO (0.025 %, V/V) was used as control. UP refers to unmethylation primers; MP refers to methylation primers. qPCR was performed with 3 biological replicates and 2 technical replicates. Similar results were obtained in human chondrocytes from 6 independent donors. * indicates P < 0.05, * ** indicates P < 0.001, and * ** * indicates P < 0.0001.