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Fig. 2 Jadomycin B inhibits proliferation of MDA-MB-231 cells in zebrafish larvae. MDA-MB-231 breast cancer cells were labelled with CellTracker™ CM-Dil (yellow) fluorescent dye and transplanted into the yolk sacs of 48 h post-fertilization casper zebrafish larvae. CM-Dil positive larvae were treated with vehicle control (1:7 MeOH:H2O), or jadomycin B at final concentrations of 10, 20, and 40 µmol/L. The fold change in MDA-MB-231 cells as determined by live cell imaging of zebrafish larvae 48 h post-treatment (hpt) (A). Error bars represent the mean ± SEM (n = 20 larvae per group). *P < 0.05 compared to the vehicle control, one-way ANOVA followed by Bonferroni multiple comparison test. Representative brightfield (left) and accompanying fluorescent (right) imaging of live larvae comparing vehicle and 40 µm jadomycin B treatment at 72 hpt and 120 hpt (B). Images are representative of 20 treated larvae per group.