Figure 2

Figure 2

Analysis of E+ fish muscles at 12 vs. 18 months. The heatmap (A) shows the top 25 metabolites separating groups and their relative abundances per sample (red represents a higher concentration per sample while blue indicates a lower one). The partial least squares-discriminant analysis (PLS-DA) scores plot (B) separates 12- and 18-month E+ muscle samples by feature differences and displays a 95% confidence region around samples. Important features plot displays variable importance in projection (VIP) (C) of the PLS-DA and provides an estimation of the discriminatory power of each metabolite with a score. Larger VIP scores indicate greater discriminatory power in the PLS-DA models. The table (D) lists the top values shown in the graph of pathway analysis, while figure (E) displays pathway analysis by ranking metabolites by p-value (shown as −log10(p)) and pathway impact referenced against the KEGG (Kiyoto Encyclopedia of Genes and Genomes) Pathway Database. Red indicates highest impacts, clear the lowest, yellow and orange are intermediate.