Figure 4.

Figure 4.

Effects of a 24-h bath applied treatments during embryonic development on spontaneous swimming of zebrafish larvae. A, Representative path reconstructions for 24 individual larvae during a 10-min trial for three experimental conditions. We performed the experiments at 6–7 dpf when the properties of swimming episodes are relatively stable in control conditions. The portion of the path corresponding to cruises episodes are shown in green, and to bursts are shown in red. B–D, Boxplots showing the mean distance per larva following ENE increase (green), in control conditions (blue), and following ENE decrease (red). Distance is expressed as the cumulated length of the path covered. B, Distance covered when no threshold is applied for the analysis. C, Distance covered during cruises. D, Distance covered during bursts. For all boxplots the p of normality test for at least one condition was <0.05; therefore, KW test was used, 192 < n < 384. ****p < 0.001.