Absence of changes in programmed cell death following modifications of ENE. A, Maximum projection of confocal z series of the brain of 6- to 7-dpf Tg(Et.vMAT2:eGFP) larvae, following ENE increase (left image, boxed in green), in control conditions (middle image, boxed in blue), and following ENE decrease (right image, boxed in red). Immunostaining to caspase-3 (magenta) and GFP (cyan) are shown as merged channels. Scale bars = 50 μm. B, Boxplots showing the number of the caspase-3+ IR cells counted in the telencephalon following ENE increase (green), in control conditions (blue) and following ENE decrease (red). KW test, 23 < n < 26 telencephalon from four independent experiments. ns = non significant.
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