ZFIN Image: Tang et al., 2023, Figure 3

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Figure 3

The expression of cMyc is activated around the injury site of zebrafish hearts, which is excessively upregulated in Tg(Δ113p53:cmyc) transgenic zebrafish injury hearts. (A) Immunostaining of MF20 (in red) and cMyc (in green) in WT sham and injury hearts at 7 and 14 dpa. Framed areas were magnified in right panels. n: 8–11 hearts/sample. Scale bar: 100 μm. (B) Diagram showing the construction of Tg(∆113p53:cmyc) transgene. (C) qRT-PCR was performed to exam the expression of cmyc in WT and Tg(∆113p53:cmyc) sham hearts. (D,E) Immunostaining of MF20 (in red) and cMyc (in green) of WT and Tg(∆113p53:cmyc) hearts at 14 dpa (D) and 30 dpa (E). Framed areas were magnified in lower panels. n: 10–12 hearts/sample. Scale bar: 100 μm. Statistical analysis was performed by Student’s two-tailed unpaired t test in GraphPad Prism 8. The p values were represented by n.s. and asterisks. n.s., p > 0.05.

Acknowledgments

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