ZFIN Image: Flex et al., 2022, Fig. 4

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Figure Caption

Fig. 4

Disease-causing KIF5B mutations affect autophagosomes morphology and subcellular localization. (A) Confocal microscopy analyses show a peculiar subcellular localization of autophagosomes in patients? cells compared to control cells. In particular, these organelles localize near the nucleus and are enlarged compared to that observed in control cells, and a few number colocalized with lysosomes residing in the peripheral region of the cell. Scale bars are respectively 10 ?m (left) and 2 ?m (right). (B) In these panels, this behavior is exacerbated by treatment with bafilomycin. Scale bars are respectively 10 ?m (left) and 5 ?m (right). The cells were stained with antibodies against Lamp1 (lysosomes marker, red), LC3I/II (autophagosomes marker, green) and DAPI (DNA marker, blue).

Acknowledgments

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