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FIG. 6.
Manipulative functionalities of our VR platform on 4D zebrafish heart models. (a) The VR platform provides users with an immersive viewing and interactive experience of a 4D zebrafish heart model, allowing us to visualize and analyze the heart function in a user-defined mode over time. (b) Overview of the analyzing tool in VR. The available interactions in the VR platform include cell selection, time point selection, time pause, and lighting adjustment. Trajectories for the selected cells are displayed as dotted lines, and a glowing point indicates the current position in the trajectory. After cell selection, the VR platform provides quantitative analysis outputs, including velocity, volume, surface area, and relative distance if two cells are selected. (c) and (d) To validate the platform's functions, we randomly selected two cells in the ventricle and atrium (VC: ventricular cell and AC: atrial cell) and compared their traces at different time points. (e)–(g) After collecting the measurement results from our VR platform, we compared the velocity and relative distance change in one cardiac cycle between selected cells, and the SA:V ratio for selected cells in different heart regions at 3 (top) and 7 dpf (bottom), respectively. (e) The average velocity changes in five ventricular cells and five atrial cells. (f) The relative distance changes between three groups of cells, i.e., two ventricular cells, a ventricular cell and an atrial cell, and two atrial cells. (g) SA:V ratio change of four randomly selected ventricular and atrial cells during one cardiac cycle are depicted over the time course.