Light-sheet imaging of GFP-labeled cardiomyocyte nuclei in the transgenic Tg(cmlc2:nucGFP) zebrafish larvae. (a) Cardiac contraction was captured from ventricular systole to diastole in 3–7 days post fertilization (dpf), respectively. (b) 4D Reconstruction of contracting hearts in zebrafish larvae at 4 (top) and 7 dpf (bottom) was presented as a maximum intensity projection (MIP) image. The clock on the upper left of each frame indicates the cardiac phase starting from end-systole. Cardiac ventricle and atrium are depicted in pink and blue, respectively. V: ventricle. A: atrium. AVC: atrioventricular canal. Scale bar: 30 μm.
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