Figure 7

Figure 7

Cell proliferation in the telencephalon of zebrafish. (A) Immunohistochemical staining against PCNA and nuclear counterstain with DAPI. From left to right: fish underwent 21 days of DMSO, 21 days of prednisolone, 17 days of DMSO followed by 4 days of prednisolone and 17 days of prednisolone followed by 4 days of DMSO treatment. Scalebar 50 µm. (B) Quantification of PCNA+ cells in the telencephalon. Data are mean ± SD of PCNA+ cells in the different groups (21 days of DMSO, 21 days of prednisolone, 17 days of DMSO followed by 4 days of prednisolone and 17 days of prednisolone followed by 4 days of DMSO. Each dot represents one biological replicate. Parametric testing because of normal distribution of the data. Statistical significance was tested by post-hoc Dunnett’s multiple comparison after one-way ANOVA. n=5 (4 females, 1 male in DMSO 21d, DMSO 17d Pred 4d, Pred 17 d DMSO 4d; 5 females in Pred 21d) in all groups with 2 sections per individual.