Figure 5

Figure 5

Quantification of PCNA positive cells along the skull bone of the zebrafish. (A) Total number of PCNA+ cells lining the skull bone. (B) Percentage of PCNA+ cells lining the skull bone (normalized to all cells lining the skull bone). Data are mean ± SD of PCNA positive cells lining bone in the different groups (21 days of DMSO, 21 days of prednisolone, 17 days of DMSO followed by 4 days of prednisolone and 17 days of prednisolone followed by 4 days of DMSO treatment). Each dot represents one biological replicate. Non-parametric testing because of non-normal distribution of the data. Statistical significance was tested by post-hoc Dunn’s multiple comparison after Kruskal-Wallis test. n=5 (4 females, 1 male in DMSO 21d, DMSO 17d Pred 4d, Pred 17 d DMSO 4d; 5 females in Pred 21d) in all groups with 1 section per individual.