Figure 2

Figure 2

Quantification of the 7xTCF-Xia.Siam:nlsmCherry Wnt reporter expression in the fin regenerate. Lines represent mean ± SEM of mCherry signal intensity for the four different treatments measured at 9 dpa, 14 dpa, 17 dpa, and 21 dpa. Signal was measured from the tip towards the amputation plane. Boxes indicate “Wnt-active zone” at 9 dpa, defined as levels beyond baseline expression levels detected at 21 dpa (i.e. point of intersection of 9 dpa curve with 21 dpa curve). n ^{(DMSO 21d,9/14/17/21dpa)} = 5 (4 females, 1 male); n ^{(Pred 21d,9dpa)} = 8 (6 females, 2 males), n ^{(Pred 21d,14dpa)} = 7 (6 females, 1 male), n ^{(Pred 21d,17/21dpa)} = 6 (6 females); n ^{(DMSO 17d Pred 4d,9/14dpa)} = 8 (6 females, 2 males), n ^{(DMSO 17d Pred 4d,17/21dpa)} = 7 (6 females, 1 male); n ^{(Pred 17d DMSO 4d,9dpa)} = 8 (6 females, 2 males), n ^{(Pred 17d DMSO 4d,14/17/21dpa)} = 7 (6 females, 1 male).