Fig. 4

Fig. 4

Fig. 4. Ntn1a, ntn1b, dcc, and unc5b are each required for normal protoglomerular targeting of or111-7 transgene-expressing OSNs. (A) The rate of misprojections in ntn1a, ntn1b, or ntn1a/ntn1b double mutants is elevated as compared to wild type embryos. There is no significant increase in errors in double mutants as compared to either single mutant alone. (Bars represent the mean number of misprojections. Circle widths are proportional to the number of samples counted with the indicated number of misprojections.) (B) Significant mistargeting to the DZ protoglomerulus is observed in ntn1a and in ntn1a/ntn1b double mutants, and to the MG protoglomerulus in ntn1b mutants. (C) Significantly increased protoglomerular and ectopic errors are observed in dcc mutants. (Bars represent the mean number of misprojections. Circle widths are proportional to the number of samples counted with the indicated number of misprojections.) (D) Significant protoglomerular mistargeting is directed towards the DZ and MG protoglomeruli in dcc mutants. (E) Significantly increased protoglomerular and ectopic errors are observed in unc5b mutants. (Bars represent the mean number of misprojections. Circle widths are proportional to the number of samples counted with the indicated number of misprojections) (F) Protoglomerular mistargeting is directed towards the DZ and MG protoglomeruli in unc5b mutants. Additional aberrant trajectories towards the midline were observed in unc5b mutants. Student’s T-test was used to assess the significance of overall error rates, while Fisher’s exact test was used to assess the significance of protoglomerular mistargeting (*: 0.05, **: 0.01, ***: 0.001; two tailed).