Fig. 5

Fig. 5 Fig. 5. Combination treatments of xenotransplanted larvae in EWS:FLI1low conditions A) Schematic representation of the workflow: shSK-E17T cells were pre-treated with doxycycline for 7 days in culture to induce knockdown of EWS:FLI1. Xenotransplanted embryos were incubated in E3 with doxycycline over the course of the experiment. Embryos were treated with A-1331852 (10 μM), S63845 (5 μM), irinotecan (5 μM), YK-4-279 (5 μM) and respective combinations for 48 h (1 dpi - 3 dpi). B) Representative images for average change in tumor size for every condition. C) Relative change of tumor area (3 dpi/1 dpi). D) Mean absolute tumor sizes and percent changes. Scale bar is 125 μm. For evaluation, single treatments were compared to DMSO control and combination treatments were compared to respective single treatments (top significance value indicates comparison to first compound, bottom value indicates comparison to second compound). Statistical analyses were performed with a Kruskal-Wallis test, ****: p ≤ 0.0001, ***: p ≤ 0.001, *: p ≤ 0.05. Error bars represent SEM of combined larvae of two independent experiments.