Figure 1

Figure 1

The knockout of glut2 in zebrafish. (A) Transcriptional expression analysis of glut2 in different tissues (n = 6). (B) Sequence comparison of glut2 alleles in the control and two glut2 knockout zebrafish lines. (C) Schematic representation of glut2 full-length putative peptide from the control and two glut2 knockout fish lines. aa, amino acids. (D) The transcriptional expression of glut2 in 5 dpf control and glut2^−/− fish in the F3 population of both lines 1 and 2 (n = 3). (E) The hatching rate analysis of the control (glut2^+/+), glut2^+/−, and glut2^−/− fish from 0–3 dpf in the F3 population of line 2 (n = 6). (F) Analysis of the control, glut2^+/−, and glut2^−/− survival rates from 6 to 21 dpf in line 2 F3 population (236 fish were genotyped for analysis). (G–I) Representative images of the overall morphology of the control, glut2^+/−, and glut2^−/− fish at 30 dpf. (J, K) Body weight and body length of the control (n = 26), glut2^+/− (n = 53), and glut2^−/− (n = 26) fish at 30 dpf. A two-tailed unpaired t-test was used to detect significance. ****P < 0.0001. ns, no significance.