Fig. 6

Fig. 6

Fig. 6. Cdx1b represses multiple signaling pathways essential for liver development. A: The effect of various signaling inhibitors on the hepatized intestine phenotype in cdx1b^zju1/zju1. Left, representative images showing the effect of the Bmp-signaling inhibitor DMH1, Fgf-signaling inhibitor SU5402, Wnt-signaling inhibitor IWR-1, Hippo-signaling inhibitor Verteporfin and TGFβ-signaling inhibitor SB505124 on the intestinal ectopic expression of fabp10a in WT (top row) and cdx1b^zju1/zju1 (lower row). Right, the intestine versus liver ratio of the fabp10a signals in cdx1b^zju1/zju1. B: The mitigation effect of Bmp inhibitor DMH1 on the hepatized intestine phenotype is dosage dependent. Left, representative WISH images; right, the intestine versus liver ratio of the fabp10a signals in cdx1b^zju1/zju1. In (A) and (B), embryos were treated with inhibitors starting from 36 hpf, respectively, and were subjected to WISH at 5 dpf with the fabp10a probe. Each spot represented one embryo. ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001. C and D: Cdx1b overexpression represses liver organogenesis. In (C), WISH showing the cdx1b expression in the Tg(hsp70l:cdx1b) embryos treated with or without heat shock. Left two panels, WISH at 32 hpf after heat shock treatment (HS) at 26 hpf for 30 min; Right two panels, WISH at 5 dpf after heat shock treatment at 4.5 dpf for 30 min. In (D), WISH comparing the expression patterns of foxa3, prox1a and gata6 at 2 dpf, and fabp10a at 5 dpf between WT and Tg(Hsp70:cdx1b) (h:cdx1b) embryos treated with or without heat shock. For heat shock samples, embryos were heat-shocked at 12 hpf and 26 hpf two time points, each for 30 min, and were then collected for WISH at 2 dpf or 5 dpf. Numbers on the right corner, number of embryos exhibiting the expression pattern (numerator) over number of embryos examined. en, endoderm; in, intestine; lv, liver; in, intestine; pc, pancreas.