Figure 3

Figure 3

Characteristics of different strains. (A) Genetic stability detection of HY9901ΔexsA deletion mutantt. M: DL2000 marker; Lane 1. The 861 bp fragment amplified from genomic DNAs of HY9901 using primer pairs of ExsA-F/ExsA-R; Lane 2. The negative result amplified from genomic DNAs of ΔexsA using primer pairs of ExsA-F/ExsA-R. (B) Growth rates of HY9901ΔexsA and wild-type strain HY9901 (wt). Aliquots of cell culture were obtained at various time points and measured for cell density at OD₆₀₀. (C) Activity of extracellaluar proteases. The extracellular protease activity was measured at OD₄₄₂. (D) Measurement of biofilm by LSCM. (a) HY9901 2.5d diagram. (b)HY9901ΔexsA 2.5d diagram. (c) HY9901 2d diagram. (d) HY9901ΔexsA 2d diagram; HY9901 Biofilm thickness: 60 ± 10 μm, HY9901ΔexsA Biofilm thickness:90 ± 20 μm. (E) Swarming diameters were measured after 24 h incubation on TSA containing 0.3% agar plates. **p < 0.01.