Figure 4
chd7?/? fish have higher respiratory rates under anesthesia than their chd7+/+ counterparts. Adult fish were anesthetized, and respiratory rates (opercular movements) were recorded at the time fish lost their response to touch (time point 1? post loss of touch response) and response to fin?pinch (time point 2? post loss of fin pinch response). Fish were then moved to a recovery tank, and respiratory rates were assessed both upon entering the recovery tank (time point 3? initial recovery tank) and just before the fish recovered the ability to swim (time point 4?10 s prior to recovery). * p < 0.05, as per one-way ANOVA, with a Tukey post-test. Error bars represent standard deviation (SD). n = 7 for chd7?/? and n = 13 for the other two groups.