Figure 5—figure supplement 4.

Figure 5—figure supplement 4. Normal eye size in vsxKO animals is observed at juvenile stages.

(a-f). Visual system histological sections stained with nuclear marker DAPI and phalloidin-Alexa488 for actin filaments from wildtype (a, c and e, n=5 for both stages) and vsxKO (b, d and f) animals at 6dpf (a, b, n=8), and 19hpf (c, e, d and f, n=6). No changes in eye size are observed but lamination in vsx mutants is severely affected at later stages compared to siblings (e, f). dpf: days post fertilization, ONL: outer nuclear layer, INL: inner nuclear layer, GCL: ganglion cell layer. Scale bar (a-d): 100 µm.