Figure 4—figure supplement 3.

Figure 4—figure supplement 3. V2 spinal cord interneurons are not affected by the mutation of Vsx TFs.

(a-d). Fluorescent in situ hybridization lateral images from 24hpf wildtype (a, b) and vsxKO (c, d) mutants using vsx1 (a, c) and tal1 (b, d) probes to visualize V2a and V2b trunk interneurons, respectively. (e). The density of vsx1 (V2a) and tal1 (V2b) positive neurons was measured in wildtype (n=5 for vsx1 and n=5 for tal1) and vsxKO (n=2 for vsx1 and n=7 for tal1) mutant trunks at 24hpf. Using an unpaired t-test, no significant changes in the number of V2a and V2b neurons was observed between wildtype and vsxKO mutant animals at this stage (p>0.05). Data is shown as mean ± SD. ns: not significant. Scale bar in (a-d): 100 µm.