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Figure 7
Impaired MTH signaling affects proliferation and apoptosis of dla+ spinal cord cells in a time-restricted manner. (A) Analysis of dla expression by fluorescent in situ hybridization (green) and colocalization with cell mitosis (phosphohistone 3 immunostaining, red) in CTRLMO) andMCT8MO embryos. (B) – Box-and-whiskers plot of quantification of the number of dla+ mitotic cells in the spinal cord at 18, 22, and 25hpf in control and MCT8MO injected embryos. (C) Analysis of dla expression by fluorescent in situ hybridization (green) and colocalization with apoptotic cell detected using a TUNEL assay (red). (D) Box-and-whiskers plot quantifying the number of dla+ apoptotic cells in the spinal cord at 18, 22, and 25hpf in control and MCT8MO injected embryos. The images present a lateral view of the spinal cord between somite 8-12, rostral is to the left, and dorsal up in all images. The scale bar represents 50 µm. Colocalization was analyzed in 2 myotome volume sections within this spinal cord region (n=10-15). Statistical significance determined by t-test: two-sample, assuming equal variances. **p<0.01.