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Figure 5.
Shown are fluorescent images of PHA/PHB cilia (phasmid tail). (A) Fluorescent images from a single copy GFP::CHE-3 (human dynein heavy chain DYNC2H1) in WT and indicated mutant backgrounds are displayed. GFP::CHE-3 accumulations within cilia and dim distal cilia staining were observed in two distinct triple mutants (wdr-31(tm10423);elmd-1;rpi-2 and wdr-31(syb1568);elmd-1;rpi-2). Scale bars: 3 μm. (B, C, D, E, F) Confocal microscopy analysis of IFT-A (IFT-140::GFP) and IFT–B complex components (OSM-6/IFT52::GFP and IFT-74::GFP) revealed differential abnormalities in the transport of IFT-A and IFT-B components in double (wdr-31;elmd-1) and triple mutants. The localization of XBX-1::mCherry (Dynein subunit) in wdr-31(syb1568);elmd-1;rpi-2 triple mutants phenocopies the dim distal cilia staining of IFT-A (IFT-140::GFP) in the wdr-31(tm10423);elmd-1;rpi-2 triple mutants. OSM-3/KIF17 Kinesin motor accumulates at the ciliary tips in wdr-31(tm10423);elmd-1 double and wdr-31(tm10423);elmd-1;rpi-2 triple mutants. Compared with double mutants, the ciliary tip staining is stronger in the triple mutants. Scale bars: 3 μm. (G) Fluorescent images from Kinesin II motor (KAP-1::GFP) revealed that the restricted middle segment localization of KAP-1 remains unchanged in wdr-31(tm10423);elmd-1;rpi-2 triple mutants. Scale bars: 3 μm. (H) Shown are the drawings of phasmid cilia (PHA/PHB sensory neurons in the tail) in WT and mutants showing ciliary accumulations.